Czech Republic Grand Prix
| Pos | Num. | Rider | Nation | Team | Bike | Time/Gap |
| 1 | 99 | Jorge LORENZO | SPA | Fiat Yamaha Team | Yamaha | 43’22.638 |
| 2 | 26 | Dani PEDROSA | SPA | Repsol Honda Team | Honda | +5.494 |
| 3 | 27 | Casey STONER | AUS | Ducati Team | Ducati | +11.426 |
| 4 | 11 | Ben SPIES | USA | Monster Yamaha Tech 3 | Yamaha | +13.723 |
| 5 | 46 | Valentino ROSSI | ITA | Fiat Yamaha Team | Yamaha | +17.930 |
| 6 | 69 | Nicky HAYDEN | USA | Ducati Team | Ducati | +26.815 |
| 7 | 5 | Colin EDWARDS | USA | Monster Yamaha Tech 3 | Yamaha | +33.396 |
| 8 | 33 | Marco MELANDRI | ITA | San Carlo Honda Gresini | Honda | +39.406 |
| 9 | 40 | Hector BARBERA | SPA | Paginas Amarillas Aspar | Ducati | +39.639 |
| 10 | 14 | Randy DE PUNIET | FRA | LCR Honda MotoGP | Honda | +40.893 |
| 11 | 58 | Marco SIMONCELLI | ITA | San Carlo Honda Gresini | Honda | +42.032 |
| 12 | 41 | Aleix ESPARGARO | SPA | Pramac Racing Team | Ducati | +47.091 |
| 13 | 15 | Alex DE ANGELIS | RSM | Interwetten Honda MotoGP | Honda | +51.368 |
| 19 | Alvaro BAUTISTA | SPA | Rizla Suzuki MotoGP | Suzuki | 1 Lap | |
| 36 | Mika KALLIO | FIN | Pramac Racing Team | Ducati | 15 Laps | |
| 4 | Andrea DOVIZIOSO | ITA | Repsol Honda Team | Honda | 16 Laps | |
| 65 | Loris CAPIROSSI | ITA | Rizla Suzuki MotoGP | Suzuki | 21 Laps |